Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach

Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach

Abstract Background The generation of point mutations is a major tool for evaluating the roles of specific nucleotides or amino acids within the regulatory or functional landscape. However, examination of these mutations in vivo requires the generation of animals carrying only the relevant point mut...

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Bibliographic Details
Main Authors: Tgst Levi, Anna Sloutskin, Rachel Kalifa, Tamar Juven-Gershon, Offer Gerlitz
Format: Article
Language:English
Published: BMC 2020-07-01
Series:Biological Procedures Online
Subjects:
CRISPR/Cas9
co-CRISPR
ssODN
HDR
Point mutations
Drosophila
Online Access:http://link.springer.com/article/10.1186/s12575-020-00123-7

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http://link.springer.com/article/10.1186/s12575-020-00123-7

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