An optimized CRISPR/Cas9 approach for precise genome editing in neurons

An optimized CRISPR/Cas9 approach for precise genome editing in neurons

The efficient knock-in of large DNA fragments to label endogenous proteins remains especially challenging in non-dividing cells such as neurons. We developed Targeted Knock-In with Two (TKIT) guides as a novel CRISPR/Cas9 based approach for efficient, and precise, genomic knock-in. Through targeting...

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Bibliographic Details
Main Authors: Huaqiang Fang, Alexei M Bygrave, Richard H Roth, Richard C Johnson, Richard L Huganir
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-03-01
Series:eLife
Subjects:
CRISPR/Cas9
genome editing
endogenous protein tagging
AMPA receptor
NMDA receptor
Online Access:https://elifesciences.org/articles/65202

Internet

https://elifesciences.org/articles/65202

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