B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of α-dystroglycan
Recent studies demonstrated that mutations in B3GNT1, an enzyme proposed to be involved in poly-N-acetyllactosamine synthesis, were causal for congenital muscular dystrophy with hypoglycosylation of α-dystroglycan (secondary dystroglycanopathies). Since defects in the O-mannosylation protein glycosy...
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doaj-f3069063686a46eea8a0c872344722b82021-05-04T23:28:29ZengeLife Sciences Publications LtdeLife2050-084X2014-10-01310.7554/eLife.03943B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of α-dystroglycanJeremy L Praissman0David H Live1Shuo Wang2Annapoorani Ramiah3Zoeisha S Chinoy4Geert-Jan Boons5Kelley W Moremen6Lance Wells7Complex Carbohydrate Research Center, University of Georgia, Athens, United States; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, United StatesComplex Carbohydrate Research Center, University of Georgia, Athens, United StatesComplex Carbohydrate Research Center, University of Georgia, Athens, United StatesComplex Carbohydrate Research Center, University of Georgia, Athens, United StatesComplex Carbohydrate Research Center, University of Georgia, Athens, United StatesComplex Carbohydrate Research Center, University of Georgia, Athens, United StatesComplex Carbohydrate Research Center, University of Georgia, Athens, United States; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, United StatesComplex Carbohydrate Research Center, University of Georgia, Athens, United States; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, United StatesRecent studies demonstrated that mutations in B3GNT1, an enzyme proposed to be involved in poly-N-acetyllactosamine synthesis, were causal for congenital muscular dystrophy with hypoglycosylation of α-dystroglycan (secondary dystroglycanopathies). Since defects in the O-mannosylation protein glycosylation pathway are primarily responsible for dystroglycanopathies and with no established O-mannose initiated structures containing a β3 linked GlcNAc known, we biochemically interrogated this human enzyme. Here we report this enzyme is not a β-1,3-N-acetylglucosaminyltransferase with catalytic activity towards β-galactose but rather a β-1,4-glucuronyltransferase, designated B4GAT1, towards both α- and β-anomers of xylose. The dual-activity LARGE enzyme is capable of extending products of B4GAT1 and we provide experimental evidence that B4GAT1 is the priming enzyme for LARGE. Our results further define the functional O-mannosylated glycan structure and indicate that B4GAT1 is involved in the initiation of the LARGE-dependent repeating disaccharide that is necessary for extracellular matrix protein binding to O-mannosylated α-dystroglycan that is lacking in secondary dystroglycanopathies.https://elifesciences.org/articles/03943congenital muscular dystrophyO-mannosylationglycosylationalpha-dystroglycanB4GAT1B3GNT1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeremy L Praissman David H Live Shuo Wang Annapoorani Ramiah Zoeisha S Chinoy Geert-Jan Boons Kelley W Moremen Lance Wells |
spellingShingle |
Jeremy L Praissman David H Live Shuo Wang Annapoorani Ramiah Zoeisha S Chinoy Geert-Jan Boons Kelley W Moremen Lance Wells B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of α-dystroglycan eLife congenital muscular dystrophy O-mannosylation glycosylation alpha-dystroglycan B4GAT1 B3GNT1 |
author_facet |
Jeremy L Praissman David H Live Shuo Wang Annapoorani Ramiah Zoeisha S Chinoy Geert-Jan Boons Kelley W Moremen Lance Wells |
author_sort |
Jeremy L Praissman |
title |
B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of α-dystroglycan |
title_short |
B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of α-dystroglycan |
title_full |
B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of α-dystroglycan |
title_fullStr |
B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of α-dystroglycan |
title_full_unstemmed |
B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of α-dystroglycan |
title_sort |
b4gat1 is the priming enzyme for the large-dependent functional glycosylation of α-dystroglycan |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2014-10-01 |
description |
Recent studies demonstrated that mutations in B3GNT1, an enzyme proposed to be involved in poly-N-acetyllactosamine synthesis, were causal for congenital muscular dystrophy with hypoglycosylation of α-dystroglycan (secondary dystroglycanopathies). Since defects in the O-mannosylation protein glycosylation pathway are primarily responsible for dystroglycanopathies and with no established O-mannose initiated structures containing a β3 linked GlcNAc known, we biochemically interrogated this human enzyme. Here we report this enzyme is not a β-1,3-N-acetylglucosaminyltransferase with catalytic activity towards β-galactose but rather a β-1,4-glucuronyltransferase, designated B4GAT1, towards both α- and β-anomers of xylose. The dual-activity LARGE enzyme is capable of extending products of B4GAT1 and we provide experimental evidence that B4GAT1 is the priming enzyme for LARGE. Our results further define the functional O-mannosylated glycan structure and indicate that B4GAT1 is involved in the initiation of the LARGE-dependent repeating disaccharide that is necessary for extracellular matrix protein binding to O-mannosylated α-dystroglycan that is lacking in secondary dystroglycanopathies. |
topic |
congenital muscular dystrophy O-mannosylation glycosylation alpha-dystroglycan B4GAT1 B3GNT1 |
url |
https://elifesciences.org/articles/03943 |
work_keys_str_mv |
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