Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

Cas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Ca...

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Main Authors:	Bo Zhang, Yangmiao Ye, Weiwei Ye, Vanja Perčulija, Han Jiang, Yiyang Chen, Yu Li, Jing Chen, Jinying Lin, Siqi Wang, Qi Chen, Yu-San Han, Songying Ouyang
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-06-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-10507-3

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spelling	doaj-f30e478b0a24490ca6dcfdd16c1b148d2021-05-11T12:39:40ZengNature Publishing GroupNature Communications2041-17232019-06-0110111110.1038/s41467-019-10507-3Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13dBo Zhang0Yangmiao Ye1Weiwei Ye2Vanja Perčulija3Han Jiang4Yiyang Chen5Yu Li6Jing Chen7Jinying Lin8Siqi Wang9Qi Chen10Yu-San Han11Songying Ouyang12The Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityInstitute of Fisheries Science, College of Life Science, National Taiwan UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityCas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Cas13d system.https://doi.org/10.1038/s41467-019-10507-3
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Bo Zhang Yangmiao Ye Weiwei Ye Vanja Perčulija Han Jiang Yiyang Chen Yu Li Jing Chen Jinying Lin Siqi Wang Qi Chen Yu-San Han Songying Ouyang
spellingShingle	Bo Zhang Yangmiao Ye Weiwei Ye Vanja Perčulija Han Jiang Yiyang Chen Yu Li Jing Chen Jinying Lin Siqi Wang Qi Chen Yu-San Han Songying Ouyang Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d Nature Communications
author_facet	Bo Zhang Yangmiao Ye Weiwei Ye Vanja Perčulija Han Jiang Yiyang Chen Yu Li Jing Chen Jinying Lin Siqi Wang Qi Chen Yu-San Han Songying Ouyang
author_sort	Bo Zhang
title	Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
title_short	Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
title_full	Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
title_fullStr	Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
title_full_unstemmed	Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
title_sort	two hepn domains dictate crispr rna maturation and target cleavage in cas13d
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-06-01
description	Cas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Cas13d system.
url	https://doi.org/10.1038/s41467-019-10507-3
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Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

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