Reversible and rapid transfer-RNA deactivation as a mechanism of translational repression in stress.
Stress-induced changes of gene expression are crucial for survival of eukaryotic cells. Regulation at the level of translation provides the necessary plasticity for immediate changes of cellular activities and protein levels. In this study, we demonstrate that exposure to oxidative stress results in...
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2013-08-01
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doaj-f337cc3bfb814f29be21dc93fdbad3ef2020-11-25T00:24:21ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042013-08-0198e100376710.1371/journal.pgen.1003767Reversible and rapid transfer-RNA deactivation as a mechanism of translational repression in stress.Andreas CzechSandra WendeMario MörlTao PanZoya IgnatovaStress-induced changes of gene expression are crucial for survival of eukaryotic cells. Regulation at the level of translation provides the necessary plasticity for immediate changes of cellular activities and protein levels. In this study, we demonstrate that exposure to oxidative stress results in a quick repression of translation by deactivation of the aminoacyl-ends of all transfer-RNA (tRNA). An oxidative-stress activated nuclease, angiogenin, cleaves first within the conserved single-stranded 3'-CCA termini of all tRNAs, thereby blocking their use in translation. This CCA deactivation is reversible and quickly repairable by the CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase]. Through this mechanism the eukaryotic cell dynamically represses and reactivates translation at low metabolic costs.http://europepmc.org/articles/PMC3757041?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andreas Czech Sandra Wende Mario Mörl Tao Pan Zoya Ignatova |
spellingShingle |
Andreas Czech Sandra Wende Mario Mörl Tao Pan Zoya Ignatova Reversible and rapid transfer-RNA deactivation as a mechanism of translational repression in stress. PLoS Genetics |
author_facet |
Andreas Czech Sandra Wende Mario Mörl Tao Pan Zoya Ignatova |
author_sort |
Andreas Czech |
title |
Reversible and rapid transfer-RNA deactivation as a mechanism of translational repression in stress. |
title_short |
Reversible and rapid transfer-RNA deactivation as a mechanism of translational repression in stress. |
title_full |
Reversible and rapid transfer-RNA deactivation as a mechanism of translational repression in stress. |
title_fullStr |
Reversible and rapid transfer-RNA deactivation as a mechanism of translational repression in stress. |
title_full_unstemmed |
Reversible and rapid transfer-RNA deactivation as a mechanism of translational repression in stress. |
title_sort |
reversible and rapid transfer-rna deactivation as a mechanism of translational repression in stress. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2013-08-01 |
description |
Stress-induced changes of gene expression are crucial for survival of eukaryotic cells. Regulation at the level of translation provides the necessary plasticity for immediate changes of cellular activities and protein levels. In this study, we demonstrate that exposure to oxidative stress results in a quick repression of translation by deactivation of the aminoacyl-ends of all transfer-RNA (tRNA). An oxidative-stress activated nuclease, angiogenin, cleaves first within the conserved single-stranded 3'-CCA termini of all tRNAs, thereby blocking their use in translation. This CCA deactivation is reversible and quickly repairable by the CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase]. Through this mechanism the eukaryotic cell dynamically represses and reactivates translation at low metabolic costs. |
url |
http://europepmc.org/articles/PMC3757041?pdf=render |
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