Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9.

Toxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the T. gondii genome remains labor intensive, and is often restricted to lab-adapted strains or lines carrying mu...

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Bibliographic Details
Main Authors: Saima M Sidik, Caroline G Hackett, Fanny Tran, Nicholas J Westwood, Sebastian Lourido
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4074098?pdf=render