IFI16 knockdown in primary HIV-1 target cells

IFI16 knockdown in primary HIV-1 target cells

Summary: IFI16 is an important player of the host intrinsic immune response. Among others, it has been reported to sense intermediate products of HIV-1 reverse transcription in the cytosol and to sequester the transcription factor Sp1 in the nucleus to attenuate viral gene expression. Here, we prese...

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Main Authors: Matteo Bosso, Caterina Prelli Bozzo, Meta Volcic, Frank Kirchhoff
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Immunology
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302239
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spelling doaj-f38345dac3c74405b238c7fe9d7735012021-03-22T12:52:44ZengElsevierSTAR Protocols2666-16672021-03-0121100236IFI16 knockdown in primary HIV-1 target cellsMatteo Bosso0Caterina Prelli Bozzo1Meta Volcic2Frank Kirchhoff3Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany; Corresponding authorInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, GermanyInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, GermanyInstitute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany; Corresponding authorSummary: IFI16 is an important player of the host intrinsic immune response. Among others, it has been reported to sense intermediate products of HIV-1 reverse transcription in the cytosol and to sequester the transcription factor Sp1 in the nucleus to attenuate viral gene expression. Here, we present three different methods to reduce IFI16 protein expression levels in HIV-1 primary target cells. These techniques can be adapted for the investigation of other cellular factors in primary macrophages and CD4+ T lymphocytes.For complete details on the use and execution of this protocol, please refer to Hotter et al. (2019) and Bosso et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720302239ImmunologyMolecular Biology
collection DOAJ
language English
format Article
sources DOAJ
author Matteo Bosso
Caterina Prelli Bozzo
Meta Volcic
Frank Kirchhoff
spellingShingle Matteo Bosso
Caterina Prelli Bozzo
Meta Volcic
Frank Kirchhoff
IFI16 knockdown in primary HIV-1 target cells
STAR Protocols
Immunology
Molecular Biology
author_facet Matteo Bosso
Caterina Prelli Bozzo
Meta Volcic
Frank Kirchhoff
author_sort Matteo Bosso
title IFI16 knockdown in primary HIV-1 target cells
title_short IFI16 knockdown in primary HIV-1 target cells
title_full IFI16 knockdown in primary HIV-1 target cells
title_fullStr IFI16 knockdown in primary HIV-1 target cells
title_full_unstemmed IFI16 knockdown in primary HIV-1 target cells
title_sort ifi16 knockdown in primary hiv-1 target cells
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-03-01
description Summary: IFI16 is an important player of the host intrinsic immune response. Among others, it has been reported to sense intermediate products of HIV-1 reverse transcription in the cytosol and to sequester the transcription factor Sp1 in the nucleus to attenuate viral gene expression. Here, we present three different methods to reduce IFI16 protein expression levels in HIV-1 primary target cells. These techniques can be adapted for the investigation of other cellular factors in primary macrophages and CD4+ T lymphocytes.For complete details on the use and execution of this protocol, please refer to Hotter et al. (2019) and Bosso et al. (2020).
topic Immunology
Molecular Biology
url http://www.sciencedirect.com/science/article/pii/S2666166720302239
work_keys_str_mv AT matteobosso ifi16knockdowninprimaryhiv1targetcells
AT caterinaprellibozzo ifi16knockdowninprimaryhiv1targetcells
AT metavolcic ifi16knockdowninprimaryhiv1targetcells
AT frankkirchhoff ifi16knockdowninprimaryhiv1targetcells
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