Expression of Aspergillus niger glucose oxidase in yeast Pichia pastoris SMD1168

Expression of Aspergillus niger glucose oxidase in yeast Pichia pastoris SMD1168

The aim of this study was to establish a system for high expression of Aspergillus niger glucose oxidase (GOD) with glyceraldehyde-3-phosphate dehydrogenase gene promoter (pGAP) in Pichia SMD1168. The GOD gene from A. niger accc30161 was inserted into pGAPZαA plasmid carrying a pGAP promoter and was...

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Bibliographic Details
Main Authors: Zhanjun Qiu, Yuanfang Guo, Xiaoming Bao, Jianrong Hao, Gaoying Sun, Bingyin Peng, Wenxiang Bi
Format: Article
Language:English
Published: Taylor & Francis Group 2016-09-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Glucose oxidase
Aspergillus niger
Pichia pastoris
protein expression
promoter
Online Access:http://dx.doi.org/10.1080/13102818.2016.1193442

Internet

http://dx.doi.org/10.1080/13102818.2016.1193442

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