Rapid high-resolution melting genotyping scheme for Escherichia coli based on MLST derived single nucleotide polymorphisms

Abstract Routinely used typing methods including MLST, rep-PCR and whole genome sequencing (WGS) are time-consuming, costly, and often low throughput. Here, we describe a novel mini-MLST scheme for Eschericha coli as an alternative method for rapid genotyping. Using the proposed mini-MLST scheme, 10...

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Bibliographic Details
Main Authors: Matej Bezdicek, Marketa Nykrynova, Karel Sedlar, Stanislava Kralova, Jana Hanslikova, Aja Komprdova, Helena Skutkova, Iva Kocmanova, Jiri Mayer, Martina Lengerova
Format: Article
Language:English
Published: Nature Publishing Group 2021-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-96148-3