Macromolecular crowding effects on the kinetics of opposing reactions catalyzed by alcohol dehydrogenase

Macromolecular crowding effects on the kinetics of opposing reactions catalyzed by alcohol dehydrogenase

In order to better understand how the complex, densely packed, heterogeneous milieu of a cell influences enzyme kinetics, we exposed opposing reactions catalyzed by yeast alcohol dehydrogenase (YADH) to both synthetic and protein crowders ranging from 10 to 550 kDa. The results reveal that the effec...

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Bibliographic Details
Main Authors: Xander E. Wilcox, Charmaine B. Chung, Kristin M. Slade
Format: Article
Language:English
Published: Elsevier 2021-07-01
Series:Biochemistry and Biophysics Reports
Subjects:
Alcohol dehydrogenase
Depletion layer
Dextran
Macromolecular crowding
Michaelis-menten
Steady state kinetics
Online Access:http://www.sciencedirect.com/science/article/pii/S2405580821000509

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http://www.sciencedirect.com/science/article/pii/S2405580821000509

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