Prevalence of strains of the Bacillus cereus group biofilm producers in ice cream in Mexico
Ice cream is a medium for microbial growth due to its nutritional value and neutral pH. Therefore, the frequency of strains of the Bacillus cereus group in ice cream was determined, the enterotoxigenic profile, psychrophilic strains and biofilm production. A total of 230 samples of six brands of ice...
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Universidad de Zulia
2019-12-01
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doaj-f437505c4a0c4928a490f6437fb8c6612020-11-25T03:33:49ZspaUniversidad de ZuliaKasmera0075-52222477-96282019-12-0147211512210.5281/zenodo.352053027329Prevalence of strains of the Bacillus cereus group biofilm producers in ice cream in MexicoRoberto Adame-Gomez0Natividad Castro Alarcón1Amalia Vences-Velázquez2Elvia Rodríguez-Bataz3Maria Cristina Santiago-Dionisio4Arturo Ramírez-Peralta5Universidad Autónoma de Guerrero. Laboratorio de Investigación en Patometabolismo Microbiano. Guerrero.Universidad Autónoma de Guerrero. Laboratorio de Investigación en Microbiología. Guerrero.Universidad Autónoma de Guerrero. Laboratorio de Investigación en Inmunobiologia y Diagnóstico Molecular. Guerrero.Universidad Autónoma de Guerrero. Laboratorio de Investigación en Parasitología. Guerrero.Universidad Autónoma de Guerrero. Laboratorio de Investigación en Análisis Microbiológicos. Guerrero.Universidad Autónoma de Guerrero. Laboratorio de Investigación en Patometabolismo Microbiano. Guerrero.Ice cream is a medium for microbial growth due to its nutritional value and neutral pH. Therefore, the frequency of strains of the Bacillus cereus group in ice cream was determined, the enterotoxigenic profile, psychrophilic strains and biofilm production. A total of 230 samples of six brands of ice cream produced and distributed nationwide were collected in Mexico. The microbiological analysis was a cold pre-enrichment and isolation of the microorganism in egg yolk agar. The strains were identified molecularly from the amplification of the topoisomerase gene (gyrB) and the enterotoxigenic profile by the amplification of conserved regions of the nheABC and hblABD operons and of the cytK gene. In addition, the production of biofilm in glass and polyvinyl chloride and psychophilia was determined. The frequency of contamination by strains of the B. cereus group was 3.6%, a positive strain was found for nheABC and five for cytK, 87.5% of the strains generated biofilm in glass and all in polyvinyl chloride, two strains were psychrophilic. In the ice cream distributed in Mexico, a low contamination by strain of the B. cereus group was found, however, the enterotoxigenic potential of the strains should not be underestimated.http://produccioncientificaluz.org/index.php/kasmera/article/view/27541bacillus cereus, factores de virulencia, helado |
collection |
DOAJ |
language |
Spanish |
format |
Article |
sources |
DOAJ |
author |
Roberto Adame-Gomez Natividad Castro Alarcón Amalia Vences-Velázquez Elvia Rodríguez-Bataz Maria Cristina Santiago-Dionisio Arturo Ramírez-Peralta |
spellingShingle |
Roberto Adame-Gomez Natividad Castro Alarcón Amalia Vences-Velázquez Elvia Rodríguez-Bataz Maria Cristina Santiago-Dionisio Arturo Ramírez-Peralta Prevalence of strains of the Bacillus cereus group biofilm producers in ice cream in Mexico Kasmera bacillus cereus, factores de virulencia, helado |
author_facet |
Roberto Adame-Gomez Natividad Castro Alarcón Amalia Vences-Velázquez Elvia Rodríguez-Bataz Maria Cristina Santiago-Dionisio Arturo Ramírez-Peralta |
author_sort |
Roberto Adame-Gomez |
title |
Prevalence of strains of the Bacillus cereus group biofilm producers in ice cream in Mexico |
title_short |
Prevalence of strains of the Bacillus cereus group biofilm producers in ice cream in Mexico |
title_full |
Prevalence of strains of the Bacillus cereus group biofilm producers in ice cream in Mexico |
title_fullStr |
Prevalence of strains of the Bacillus cereus group biofilm producers in ice cream in Mexico |
title_full_unstemmed |
Prevalence of strains of the Bacillus cereus group biofilm producers in ice cream in Mexico |
title_sort |
prevalence of strains of the bacillus cereus group biofilm producers in ice cream in mexico |
publisher |
Universidad de Zulia |
series |
Kasmera |
issn |
0075-5222 2477-9628 |
publishDate |
2019-12-01 |
description |
Ice cream is a medium for microbial growth due to its nutritional value and neutral pH. Therefore, the frequency of strains of the Bacillus cereus group in ice cream was determined, the enterotoxigenic profile, psychrophilic strains and biofilm production. A total of 230 samples of six brands of ice cream produced and distributed nationwide were collected in Mexico. The microbiological analysis was a cold pre-enrichment and isolation of the microorganism in egg yolk agar. The strains were identified molecularly from the amplification of the topoisomerase gene (gyrB) and the enterotoxigenic profile by the amplification of conserved regions of the nheABC and hblABD operons and of the cytK gene. In addition, the production of biofilm in glass and polyvinyl chloride and psychophilia was determined. The frequency of contamination by strains of the B. cereus group was 3.6%, a positive strain was found for nheABC and five for cytK, 87.5% of the strains generated biofilm in glass and all in polyvinyl chloride, two strains were psychrophilic. In the ice cream distributed in Mexico, a low contamination by strain of the B. cereus group was found, however, the enterotoxigenic potential of the strains should not be underestimated. |
topic |
bacillus cereus, factores de virulencia, helado |
url |
http://produccioncientificaluz.org/index.php/kasmera/article/view/27541 |
work_keys_str_mv |
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