B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade

B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade

Summary: Helminth infection is known for generating large amounts of poly-specific IgE. Here we demonstrate that innate-like B1 cells are responsible for this IgE production during infection with the nematode parasites Nippostrongylus brasiliensis and Heligmosomoides polygyrus bakeri. In vitro analy...

Full description

Bibliographic Details
Main Authors: Rebecca K. Martin, Sheela R. Damle, Yolander A. Valentine, Matthew P. Zellner, Briana N. James, Joseph C. Lownik, Andrea J. Luker, Elijah H. Davis, Martha M. DeMeules, Laura M. Khandjian, Fred D. Finkelman, Joseph F. Urban, Jr., Daniel H. Conrad
Format: Article
Language:English
Published: Elsevier 2018-02-01
Series:Cell Reports
Subjects:
helminth
B1 cells
B2 cells
mast cells
Th2
N. brasiliensis
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124718300809
id doaj-f4577ef0704b41a9a257d4dd0468027d
record_format Article
spelling doaj-f4577ef0704b41a9a257d4dd0468027d2020-11-25T03:49:23ZengElsevierCell Reports2211-12472018-02-012271824183410.1016/j.celrep.2018.01.048B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE BlockadeRebecca K. Martin0Sheela R. Damle1Yolander A. Valentine2Matthew P. Zellner3Briana N. James4Joseph C. Lownik5Andrea J. Luker6Elijah H. Davis7Martha M. DeMeules8Laura M. Khandjian9Fred D. Finkelman10Joseph F. Urban, Jr.11Daniel H. Conrad12Department of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USADepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USADepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USADepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USADepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USADepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA; Center for Clinical and Translational Research, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USADepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USADepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USADepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA; University of Wisconsin-Madison, Madison, WI 53715, USADepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USADivision of Immunology, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA; Medicine Service, Veterans Administration Medical Center, Cincinnati, OH, USAUnited States Department of Agriculture, Agricultural Research Service, Diet, Genomics and Immunology Laboratory, Beltsville, MD 20705, USADepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA; Corresponding authorSummary: Helminth infection is known for generating large amounts of poly-specific IgE. Here we demonstrate that innate-like B1 cells are responsible for this IgE production during infection with the nematode parasites Nippostrongylus brasiliensis and Heligmosomoides polygyrus bakeri. In vitro analysis of B1 cell immunoglobulin class switch recombination to IgE demonstrated a requirement for anti-CD40 and IL-4 that was further enhanced when IL-5 was added or when the B1 source was helminth infected mice. An IL-25-induced upregulation of IgE in B1 cells was also demonstrated. In T cell-reconstituted RAG1−/− mice, N. brasiliensis clearance was enhanced with the addition of B2 cells in an IgE-dependent manner. This enhanced clearance was impeded by reconstitution with IgE sufficient B1 cells. Mucosal mast cells mediated the B2 cell enhancement of clearance in the absence of B1 cells. The data support B1 cell IgE secretion as a regulatory response exploited by the helminth.http://www.sciencedirect.com/science/article/pii/S2211124718300809helminthB1 cellsB2 cellsmast cellsTh2N. brasiliensis
collection DOAJ
language English
format Article
sources DOAJ
author Rebecca K. Martin
Sheela R. Damle
Yolander A. Valentine
Matthew P. Zellner
Briana N. James
Joseph C. Lownik
Andrea J. Luker
Elijah H. Davis
Martha M. DeMeules
Laura M. Khandjian
Fred D. Finkelman
Joseph F. Urban, Jr.
Daniel H. Conrad
spellingShingle Rebecca K. Martin
Sheela R. Damle
Yolander A. Valentine
Matthew P. Zellner
Briana N. James
Joseph C. Lownik
Andrea J. Luker
Elijah H. Davis
Martha M. DeMeules
Laura M. Khandjian
Fred D. Finkelman
Joseph F. Urban, Jr.
Daniel H. Conrad
B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade
Cell Reports
helminth
B1 cells
B2 cells
mast cells
Th2
N. brasiliensis
author_facet Rebecca K. Martin
Sheela R. Damle
Yolander A. Valentine
Matthew P. Zellner
Briana N. James
Joseph C. Lownik
Andrea J. Luker
Elijah H. Davis
Martha M. DeMeules
Laura M. Khandjian
Fred D. Finkelman
Joseph F. Urban, Jr.
Daniel H. Conrad
author_sort Rebecca K. Martin
title B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade
title_short B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade
title_full B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade
title_fullStr B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade
title_full_unstemmed B1 Cell IgE Impedes Mast Cell-Mediated Enhancement of Parasite Expulsion through B2 IgE Blockade
title_sort b1 cell ige impedes mast cell-mediated enhancement of parasite expulsion through b2 ige blockade
publisher Elsevier
series Cell Reports
issn 2211-1247
publishDate 2018-02-01
description Summary: Helminth infection is known for generating large amounts of poly-specific IgE. Here we demonstrate that innate-like B1 cells are responsible for this IgE production during infection with the nematode parasites Nippostrongylus brasiliensis and Heligmosomoides polygyrus bakeri. In vitro analysis of B1 cell immunoglobulin class switch recombination to IgE demonstrated a requirement for anti-CD40 and IL-4 that was further enhanced when IL-5 was added or when the B1 source was helminth infected mice. An IL-25-induced upregulation of IgE in B1 cells was also demonstrated. In T cell-reconstituted RAG1−/− mice, N. brasiliensis clearance was enhanced with the addition of B2 cells in an IgE-dependent manner. This enhanced clearance was impeded by reconstitution with IgE sufficient B1 cells. Mucosal mast cells mediated the B2 cell enhancement of clearance in the absence of B1 cells. The data support B1 cell IgE secretion as a regulatory response exploited by the helminth.
topic helminth
B1 cells
B2 cells
mast cells
Th2
N. brasiliensis
url http://www.sciencedirect.com/science/article/pii/S2211124718300809
work_keys_str_mv AT rebeccakmartin b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT sheelardamle b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT yolanderavalentine b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT matthewpzellner b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT briananjames b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT josephclownik b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT andreajluker b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT elijahhdavis b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT marthamdemeules b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT lauramkhandjian b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT freddfinkelman b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT josephfurbanjr b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
AT danielhconrad b1celligeimpedesmastcellmediatedenhancementofparasiteexpulsionthroughb2igeblockade
_version_ 1724495815005700096

Similar Items