Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

Abstract Background The state-of-the-art in nucleic acid based biodetection continues to be polymerase chain reaction (PCR), and many real-time PCR assays targeting biodefense pathogens for biosurveillance are in widespread use. These assays are predominantly singleplex; i.e. one assay tests for the...

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Bibliographic Details
Main Authors: Robert Player, Kathleen Verratti, Andrea Staab, Christopher Bradburne, Sarah Grady, Bruce Goodwin, Shanmuga Sozhamannan
Format: Article
Language:English
Published: BMC 2020-02-01
Series:BMC Genomics
Subjects:
Biodefense
Biodetection
Biosurveillance
Oxford Nanopore sequencing
Real-time PCR
High throughput PCR assay
Online Access:http://link.springer.com/article/10.1186/s12864-020-6557-5

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http://link.springer.com/article/10.1186/s12864-020-6557-5

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