Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations

Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations

Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of the above...

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Bibliographic Details
Main Authors: Masahiro Sato, Miyu Koriyama, Satoshi Watanabe, Masato Ohtsuka, Takayuki Sakurai, Emi Inada, Issei Saitoh, Shingo Nakamura, Kazuchika Miyoshi
Format: Article
Language:English
Published: MDPI AG 2015-08-01
Series:International Journal of Molecular Sciences
Subjects:
α-Gal epitope
α-1,3-galactosyltransferase
CRISPR/Cas9
microinjection
mRNA
mosaicism
biallelic KO
indel mutations
isolectin BS-I-B4
Online Access:http://www.mdpi.com/1422-0067/16/8/17838

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http://www.mdpi.com/1422-0067/16/8/17838

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