FRET-based identification of mRNAs undergoing translation.

We present proof-of-concept in vitro results demonstrating the feasibility of using single molecule fluorescence resonance energy transfer (smFRET) measurements to distinguish, in real time, between individual ribosomes programmed with several different, short mRNAs. For these measurements we use ei...

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Bibliographic Details
Main Authors: Benjamin Stevens, Chunlai Chen, Ian Farrell, Haibo Zhang, Jaskiran Kaur, Steven L Broitman, Zeev Smilansky, Barry S Cooperman, Yale E Goldman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3365013?pdf=render