Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors

Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors

The CRISPR-Cas9 system is used for genome editing in mammalian cells by introducing double-strand breaks (DSBs) which are predominantly repaired via non-homologous end joining (NHEJ) or to lesser extent by homology-directed repair (HDR). To enhance HDR for improving the introduction of precise genet...

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Bibliographic Details
Main Authors: Ngoc-Tung Tran, Sanum Bashir, Xun Li, Jana Rossius, Van Trung Chu, Klaus Rajewsky, Ralf Kühn
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Genetics
Subjects:
Cas9
gene editing
homologous recombination
CtIP
CRISPR
Online Access:https://www.frontiersin.org/article/10.3389/fgene.2019.00365/full

Internet

https://www.frontiersin.org/article/10.3389/fgene.2019.00365/full

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