Screening and Detecting Salmonella in Different Food Matrices in Southern Tunisia Using a Combined Enrichment/Real-Time PCR Method: Correlation with Conventional Culture Method

Screening and Detecting Salmonella in Different Food Matrices in Southern Tunisia Using a Combined Enrichment/Real-Time PCR Method: Correlation with Conventional Culture Method

A combined enrichment/ newly developed invA TaqMan® real-time PCR (qPCR) method as a screening assay to detect Salmonella spp. in 500 naturally food matrices is evaluated. DNA template for qPCR was extracted from an overnight pre-enriched sample in buffered peptone water using lysis–guanidine isothi...

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Bibliographic Details
Main Authors: Mariam Siala, Amina Barbana, Salma Smaoui, Salma Hachicha, Chema Marouane, Sana Kammoun, Radhouane Gdoura, Férièle Messadi-Akrout
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-12-01
Series:Frontiers in Microbiology
Subjects:
Salmonella
invA qPCR
prevalence
detection
different food matrices
serotype
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.02416/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2017.02416/full

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