The Ca2+ sensor protein swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons.
Swiprosin-1/EFhd2 (EFhd2) is a cytoskeletal Ca2+ sensor protein strongly expressed in the brain. It has been shown to interact with mutant tau, which can promote neurodegeneration, but nothing is known about the physiological function of EFhd2 in the nervous system. To elucidate this question, we an...
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doaj-f533f03a81a449519cee1e7649c549672020-11-25T01:31:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10397610.1371/journal.pone.0103976The Ca2+ sensor protein swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons.Pavitra PurohitFrancesc Perez-BranguliIryna ProtsEva BorgerFrank Gunn-MooreOliver WelzelKristina LoyEva Maria WenzelTeja W GrömerSebastian BrachsMax HolzerRolf BusleiKristin FritschMartin RegensburgerKonrad J BöhmBeate WinnerDirk MielenzSwiprosin-1/EFhd2 (EFhd2) is a cytoskeletal Ca2+ sensor protein strongly expressed in the brain. It has been shown to interact with mutant tau, which can promote neurodegeneration, but nothing is known about the physiological function of EFhd2 in the nervous system. To elucidate this question, we analyzed EFhd2-/-/lacZ reporter mice and showed that lacZ was strongly expressed in the cortex, the dentate gyrus, the CA1 and CA2 regions of the hippocampus, the thalamus, and the olfactory bulb. Immunohistochemistry and western blotting confirmed this pattern and revealed expression of EFhd2 during neuronal maturation. In cortical neurons, EFhd2 was detected in neurites marked by MAP2 and co-localized with pre- and post-synaptic markers. Approximately one third of EFhd2 associated with a biochemically isolated synaptosome preparation. There, EFhd2 was mostly confined to the cytosolic and plasma membrane fractions. Both synaptic endocytosis and exocytosis in primary hippocampal EFhd2-/- neurons were unaltered but transport of synaptophysin-GFP containing vesicles was enhanced in EFhd2-/- primary hippocampal neurons, and notably, EFhd2 inhibited kinesin mediated microtubule gliding. Therefore, we found that EFhd2 is a neuronal protein that interferes with kinesin-mediated transport.http://europepmc.org/articles/PMC4136728?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pavitra Purohit Francesc Perez-Branguli Iryna Prots Eva Borger Frank Gunn-Moore Oliver Welzel Kristina Loy Eva Maria Wenzel Teja W Grömer Sebastian Brachs Max Holzer Rolf Buslei Kristin Fritsch Martin Regensburger Konrad J Böhm Beate Winner Dirk Mielenz |
spellingShingle |
Pavitra Purohit Francesc Perez-Branguli Iryna Prots Eva Borger Frank Gunn-Moore Oliver Welzel Kristina Loy Eva Maria Wenzel Teja W Grömer Sebastian Brachs Max Holzer Rolf Buslei Kristin Fritsch Martin Regensburger Konrad J Böhm Beate Winner Dirk Mielenz The Ca2+ sensor protein swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons. PLoS ONE |
author_facet |
Pavitra Purohit Francesc Perez-Branguli Iryna Prots Eva Borger Frank Gunn-Moore Oliver Welzel Kristina Loy Eva Maria Wenzel Teja W Grömer Sebastian Brachs Max Holzer Rolf Buslei Kristin Fritsch Martin Regensburger Konrad J Böhm Beate Winner Dirk Mielenz |
author_sort |
Pavitra Purohit |
title |
The Ca2+ sensor protein swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons. |
title_short |
The Ca2+ sensor protein swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons. |
title_full |
The Ca2+ sensor protein swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons. |
title_fullStr |
The Ca2+ sensor protein swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons. |
title_full_unstemmed |
The Ca2+ sensor protein swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons. |
title_sort |
ca2+ sensor protein swiprosin-1/efhd2 is present in neurites and involved in kinesin-mediated transport in neurons. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Swiprosin-1/EFhd2 (EFhd2) is a cytoskeletal Ca2+ sensor protein strongly expressed in the brain. It has been shown to interact with mutant tau, which can promote neurodegeneration, but nothing is known about the physiological function of EFhd2 in the nervous system. To elucidate this question, we analyzed EFhd2-/-/lacZ reporter mice and showed that lacZ was strongly expressed in the cortex, the dentate gyrus, the CA1 and CA2 regions of the hippocampus, the thalamus, and the olfactory bulb. Immunohistochemistry and western blotting confirmed this pattern and revealed expression of EFhd2 during neuronal maturation. In cortical neurons, EFhd2 was detected in neurites marked by MAP2 and co-localized with pre- and post-synaptic markers. Approximately one third of EFhd2 associated with a biochemically isolated synaptosome preparation. There, EFhd2 was mostly confined to the cytosolic and plasma membrane fractions. Both synaptic endocytosis and exocytosis in primary hippocampal EFhd2-/- neurons were unaltered but transport of synaptophysin-GFP containing vesicles was enhanced in EFhd2-/- primary hippocampal neurons, and notably, EFhd2 inhibited kinesin mediated microtubule gliding. Therefore, we found that EFhd2 is a neuronal protein that interferes with kinesin-mediated transport. |
url |
http://europepmc.org/articles/PMC4136728?pdf=render |
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