Expression of constitutively activated Akt in the mammary gland leads to excess lipid synthesis during pregnancy and lactation
Expression of constitutively activated Akt in the mammary glands of transgenic mice results in a delay in post-lactational involution. We now report precocious lipid accumulation in the alveolar epithelium of mouse mammary tumor virus-myr-Akt transgenic mice accompanied by a lactation defect that re...
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Language: | English |
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Elsevier
2003-06-01
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Series: | Journal of Lipid Research |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520311111 |
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doaj-f57d3edb4fc2476ba8283f94891de89d |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kathryn L. Schwertfeger James L. McManaman Carol A. Palmer Margaret C. Neville Steven M. Anderson |
spellingShingle |
Kathryn L. Schwertfeger James L. McManaman Carol A. Palmer Margaret C. Neville Steven M. Anderson Expression of constitutively activated Akt in the mammary gland leads to excess lipid synthesis during pregnancy and lactation Journal of Lipid Research transgenic mice glucose transport milk fat globule lipid biosynthesis |
author_facet |
Kathryn L. Schwertfeger James L. McManaman Carol A. Palmer Margaret C. Neville Steven M. Anderson |
author_sort |
Kathryn L. Schwertfeger |
title |
Expression of constitutively activated Akt in the mammary gland leads to excess lipid synthesis during pregnancy and lactation |
title_short |
Expression of constitutively activated Akt in the mammary gland leads to excess lipid synthesis during pregnancy and lactation |
title_full |
Expression of constitutively activated Akt in the mammary gland leads to excess lipid synthesis during pregnancy and lactation |
title_fullStr |
Expression of constitutively activated Akt in the mammary gland leads to excess lipid synthesis during pregnancy and lactation |
title_full_unstemmed |
Expression of constitutively activated Akt in the mammary gland leads to excess lipid synthesis during pregnancy and lactation |
title_sort |
expression of constitutively activated akt in the mammary gland leads to excess lipid synthesis during pregnancy and lactation |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2003-06-01 |
description |
Expression of constitutively activated Akt in the mammary glands of transgenic mice results in a delay in post-lactational involution. We now report precocious lipid accumulation in the alveolar epithelium of mouse mammary tumor virus-myr-Akt transgenic mice accompanied by a lactation defect that results in a 50% decrease in litter weight over the first 9 days of lactation. Although ductal structures and alveolar units develop normally during pregnancy, cytoplasmic lipid droplets appeared precociously in mammary epithelial cells in early pregnancy and were accompanied by increased expression of adipophilin, which is associated with lipid droplets. By late pregnancy the lipid droplets had become significantly larger than in nontransgenic mice, and they persisted into lactation. The fat content of milk from lactating myr-Akt transgenic mice was 65–70% by volume compared to 25–30% in wild-type mice. The diminished growth of pups nursed by transgenic mothers could result from the high viscosity of the milk and the inability of the pups to remove sufficient quantities of milk by suckling.Transduction of the CIT3 mammary epithelial cell line with a recombinant human adenovirus encoding myr-Akt resulted in an increase in glucose transport and lipid biosynthesis, suggesting that Akt plays an important role in regulation of lipid metabolism. |
topic |
transgenic mice glucose transport milk fat globule lipid biosynthesis |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520311111 |
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doaj-f57d3edb4fc2476ba8283f94891de89d2021-04-27T04:38:51ZengElsevierJournal of Lipid Research0022-22752003-06-0144611001112Expression of constitutively activated Akt in the mammary gland leads to excess lipid synthesis during pregnancy and lactationKathryn L. Schwertfeger0James L. McManaman1Carol A. Palmer2Margaret C. Neville3Steven M. Anderson4Departments of Pathology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Obstetrics and Gynecology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Physiology and Biophysics, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Program in Molecular Biology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262Departments of Pathology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Obstetrics and Gynecology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Physiology and Biophysics, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Program in Molecular Biology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262Departments of Pathology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Obstetrics and Gynecology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Physiology and Biophysics, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Program in Molecular Biology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262Departments of Pathology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Obstetrics and Gynecology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Physiology and Biophysics, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Program in Molecular Biology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262Departments of Pathology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Obstetrics and Gynecology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Physiology and Biophysics, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262; Program in Molecular Biology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262Expression of constitutively activated Akt in the mammary glands of transgenic mice results in a delay in post-lactational involution. We now report precocious lipid accumulation in the alveolar epithelium of mouse mammary tumor virus-myr-Akt transgenic mice accompanied by a lactation defect that results in a 50% decrease in litter weight over the first 9 days of lactation. Although ductal structures and alveolar units develop normally during pregnancy, cytoplasmic lipid droplets appeared precociously in mammary epithelial cells in early pregnancy and were accompanied by increased expression of adipophilin, which is associated with lipid droplets. By late pregnancy the lipid droplets had become significantly larger than in nontransgenic mice, and they persisted into lactation. The fat content of milk from lactating myr-Akt transgenic mice was 65–70% by volume compared to 25–30% in wild-type mice. The diminished growth of pups nursed by transgenic mothers could result from the high viscosity of the milk and the inability of the pups to remove sufficient quantities of milk by suckling.Transduction of the CIT3 mammary epithelial cell line with a recombinant human adenovirus encoding myr-Akt resulted in an increase in glucose transport and lipid biosynthesis, suggesting that Akt plays an important role in regulation of lipid metabolism.http://www.sciencedirect.com/science/article/pii/S0022227520311111transgenic miceglucose transportmilk fat globulelipid biosynthesis |