An improved method for quantification of cholesterol and cholesteryl esters in human monocyte-derived macrophages by high performance liquid chromatography with identification of unassigned cholesteryl ester species by means of secondary ion mass spectrometry

The measurement of cholesteryl esters in human monocyte-derived macrophages using previously described high performance liquid chromatography methods is hampered by the presence in these cells of large amounts of triglycerides. We present a simple reversed phase high performance liquid chromatograph...

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Bibliographic Details
Main Authors: P Cullen, M Fobker, K Tegelkamp, K Meyer, F Kannenberg, A Cignarella, A Benninghoven, G Assmann
Format: Article
Language:English
Published: Elsevier 1997-02-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520374514