Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds

Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds

Efficient elimination of the editing machinery remains a challenge in plant biotechnology after genome editing to minimize the probability of off-target mutations, but it is also important to deliver end users with edited plants free of foreign DNA. Using the modular cloning system Golden Braid, we...

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Bibliographic Details
Main Authors: Norma Aliaga-Franco, Cunjin Zhang, Silvia Presa, Anjil K. Srivastava, Antonio Granell, David Alabadí, Ari Sadanandom, Miguel A. Blázquez, Eugenio G. Minguet
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-09-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9
genome editing
DsRED
Solanum lycopersicum
Oryza sativa
Arabidopsis thaliana
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2019.01150/full

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https://www.frontiersin.org/article/10.3389/fpls.2019.01150/full

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