Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathway
Gentianella acuta (Michx.) Hulten (G. acuta) has been widely used in Mongolian medicines for the treatment of cardiovascular diseases in Ewenki and Oroqen, Inner Mongolia autonomous region, China. The aim of this study was to investigate the effects and related mechanism of G. acuta on isoproterenol...
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doaj-f6610edb8111443595ab1aa63a205c562021-05-20T07:36:29ZengElsevierBiomedicine & Pharmacotherapy0753-33222019-02-01110733741Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathwayAi-Ying Li0Jing-Jing Wang1Sheng-Chang Yang2Ya-Shuo Zhao3Jie-Ru Li4Yu Liu5Jia-Huan Sun6Li-Ping An7Peng Guan8En-Sheng Ji9Hebei key laboratory of Chinese medicine research on cardio-cerebrovascular disease and Department of Biochemistry and Molecular Biology, College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR ChinaDepartment of Physiology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR ChinaDepartment of Physiology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR ChinaScientific Research Center, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR ChinaDepartment of Physiology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR ChinaHebei key laboratory of Chinese medicine research on cardio-cerebrovascular disease and Department of Biochemistry and Molecular Biology, College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR ChinaHebei key laboratory of Chinese medicine research on cardio-cerebrovascular disease and Department of Biochemistry and Molecular Biology, College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR ChinaHebei key laboratory of Chinese medicine research on cardio-cerebrovascular disease and Department of Biochemistry and Molecular Biology, College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR ChinaDepartment of Physiology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR ChinaDepartment of Physiology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR China; Corresponding author.Gentianella acuta (Michx.) Hulten (G. acuta) has been widely used in Mongolian medicines for the treatment of cardiovascular diseases in Ewenki and Oroqen, Inner Mongolia autonomous region, China. The aim of this study was to investigate the effects and related mechanism of G. acuta on isoproterenol (ISO)-induced oxidative stress, fibrosis, and myocardial damage in rats. Male Sprague Dawley rats were randomly divided into the normal control group, ISO induced group and ISO+G. acuta treatment group. Rats were administered with ISO subcutaneously (5 mg/kg/day) for 7 days, and were orally administered simultaneously with aqueous extracts of G. acuta for 21 days. This investigation showed G. acuta treatment ameliorated cardiac structural disorder, excessive collagenous fiber accumulation and cardiac malfunction. Compared with the ISO induced model group, G. acuta treatment increased superoxide dismutase (SOD) activities and glutathione (GSH) level, prevented the rise of malondialdehyde (MDA), and decreased hydroxyproline contents in the heart tissues. Moreover, G. acuta reduced the expression of transforming growth factor β1 (TGF-β1) and connective tissue growth factor (CTGF), and inhibited the expression and activation of NF-κB-P65 in myocardial tissues. These results suggested that G. acuta protects against ISO-induced cardiac malfunction probably by preventing oxidative stress, and fibrosis, and the mechanism might be through inhibiting NF-κB pathway.http://www.sciencedirect.com/science/article/pii/S0753332218369981Gentianella acutaMyocardial fibrosisNF-κBTGF-β1CollagenOxidative stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ai-Ying Li Jing-Jing Wang Sheng-Chang Yang Ya-Shuo Zhao Jie-Ru Li Yu Liu Jia-Huan Sun Li-Ping An Peng Guan En-Sheng Ji |
spellingShingle |
Ai-Ying Li Jing-Jing Wang Sheng-Chang Yang Ya-Shuo Zhao Jie-Ru Li Yu Liu Jia-Huan Sun Li-Ping An Peng Guan En-Sheng Ji Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathway Biomedicine & Pharmacotherapy Gentianella acuta Myocardial fibrosis NF-κB TGF-β1 Collagen Oxidative stress |
author_facet |
Ai-Ying Li Jing-Jing Wang Sheng-Chang Yang Ya-Shuo Zhao Jie-Ru Li Yu Liu Jia-Huan Sun Li-Ping An Peng Guan En-Sheng Ji |
author_sort |
Ai-Ying Li |
title |
Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathway |
title_short |
Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathway |
title_full |
Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathway |
title_fullStr |
Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathway |
title_full_unstemmed |
Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathway |
title_sort |
protective role of gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of nf-κb pathway |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2019-02-01 |
description |
Gentianella acuta (Michx.) Hulten (G. acuta) has been widely used in Mongolian medicines for the treatment of cardiovascular diseases in Ewenki and Oroqen, Inner Mongolia autonomous region, China. The aim of this study was to investigate the effects and related mechanism of G. acuta on isoproterenol (ISO)-induced oxidative stress, fibrosis, and myocardial damage in rats. Male Sprague Dawley rats were randomly divided into the normal control group, ISO induced group and ISO+G. acuta treatment group. Rats were administered with ISO subcutaneously (5 mg/kg/day) for 7 days, and were orally administered simultaneously with aqueous extracts of G. acuta for 21 days. This investigation showed G. acuta treatment ameliorated cardiac structural disorder, excessive collagenous fiber accumulation and cardiac malfunction. Compared with the ISO induced model group, G. acuta treatment increased superoxide dismutase (SOD) activities and glutathione (GSH) level, prevented the rise of malondialdehyde (MDA), and decreased hydroxyproline contents in the heart tissues. Moreover, G. acuta reduced the expression of transforming growth factor β1 (TGF-β1) and connective tissue growth factor (CTGF), and inhibited the expression and activation of NF-κB-P65 in myocardial tissues. These results suggested that G. acuta protects against ISO-induced cardiac malfunction probably by preventing oxidative stress, and fibrosis, and the mechanism might be through inhibiting NF-κB pathway. |
topic |
Gentianella acuta Myocardial fibrosis NF-κB TGF-β1 Collagen Oxidative stress |
url |
http://www.sciencedirect.com/science/article/pii/S0753332218369981 |
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