The Impact of IPTG Induction on Plasmid Stability and Heterologous Protein Expression by <i>Escherichia coli</i> Biofilms

The Impact of IPTG Induction on Plasmid Stability and Heterologous Protein Expression by <i>Escherichia coli</i> Biofilms

This work assesses the effect of chemical induction with isopropyl &#946;-D-1-thiogalactopyranoside (IPTG) on the expression of enhanced green fluorescent protein (eGFP) by planktonic and biofilm cells of <i>Escherichia coli</i> JM109(DE3) transformed with a plasmid containing a T7 p...

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Bibliographic Details
Main Authors: Luciana Gomes, Gabriel Monteiro, Filipe Mergulhão
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:International Journal of Molecular Sciences
Subjects:
biofilm
<i>escherichia coli</i>
heterologous protein expression
plasmid stability
plasmid copy number
iptg
Online Access:https://www.mdpi.com/1422-0067/21/2/576

Internet

https://www.mdpi.com/1422-0067/21/2/576

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