The importance of selecting the appropriate reference genes for quantitative real time PCR as illustrated using colon cancer cells and tissue [version 2; referees: 2 approved]
Quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) remains the most sensitive technique for nucleic acid quantification. Its popularity is reflected in the remarkable number of publications reporting RT-qPCR data. Careful normalisation within RT-qPCR studies is imperati...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
F1000 Research Ltd
2016-03-01
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| Series: | F1000Research |
| Subjects: | |
| Online Access: | http://f1000research.com/articles/5-99/v2 |