Expression, Identification and Purification of Dictyostelium Acetoacetyl-CoA Thiolase Expressed in Escherichia coli

<p>Acetoacetyl-CoA thiolase (AT) is an enzyme that catalyses the CoA-dependent thiolytic cleavage of acetoacetyl-CoA to yield 2 molecules of acetyl-CoA, or the reverse condensation reaction. A full-length cDNA clone pBSGT-3, which has homology to known thiolases, was isolated from<i> Dic...

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Bibliographic Details
Main Author: Takeshi Tanaka, Yasuyuki Shima, Naoki Ogawa, Koki Nagayama, Takashi Yoshida, Tetsuo Ohmachi
Format: Article
Language:English
Published: Ivyspring International Publisher 2011-01-01
Series:International Journal of Biological Sciences
Online Access:http://www.biolsci.org/v07p0009.htm