An intrabody specific for the nucleophosmin carboxy-terminal mutant and fused to a nuclear localization sequence binds its antigen but fails to relocate it in the nucleus

An intrabody specific for the nucleophosmin carboxy-terminal mutant and fused to a nuclear localization sequence binds its antigen but fails to relocate it in the nucleus

The cytoplasmic accumulation of NPM1 (NPMc+) is found in acute myeloid leukemia (AML) with NPM1 mutation. NPM1 must shuttle between nucleus and cytoplasm to assure physiological protein synthesis and, therefore, the elimination of NPMc+ is not a suitable therapeutic option. We isolated, characterize...

Full description

Bibliographic Details
Main Authors: Chiara Martinelli, Emanuela Colombo, Daniele Piccini, Cristina Sironi, Pier Giuseppe Pelicci, Ario de Marco
Format: Article
Language:English
Published: Elsevier 2014-09-01
Series:Biotechnology Reports
Subjects:
Intrabody
Leukemia
Protein shuttling
Recombinant fusion protein
Single-chain antibody
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X14000113

Internet

http://www.sciencedirect.com/science/article/pii/S2215017X14000113

Similar Items