A rapid subtractive immunization method to prepare discriminatory monoclonal antibodies for food E. coli O157:H7 contamination.

A rapid subtractive immunization method to prepare discriminatory monoclonal antibodies for food E. coli O157:H7 contamination.

To detect food E. coli O157:H7 contamination rapidly and accurately, it is essential to prepare high specific monoclonal antibodies (mAbs) against the pathogen. Cyclophosphamide (Cy)-mediated subtractive immunization strategy was performed in mice to generate mAbs that react with E. coli O157:H7, bu...

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Bibliographic Details
Main Authors: Min Jin, Jing Lang, Zhi-Qiang Shen, Zhao-Li Chen, Zhi-Gang Qiu, Xin-Wei Wang, Jun-Wen Li
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3274518?pdf=render

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http://europepmc.org/articles/PMC3274518?pdf=render

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