The Application of Dextran Sedimentation as an Initial Step in Neutrophil Purification Promotes Their Stimulation, due to the Presence of Monocytes
The purification of human neutrophils for in vitro studies is challenging as they are easily activated through ex vivo manipulations. The technique of erythrocyte sedimentation combined with density gradient centrifugation remains widely practiced and was the subject of this study. Since in the sedi...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2017-01-01
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Series: | Journal of Immunology Research |
Online Access: | http://dx.doi.org/10.1155/2017/1254792 |