Activation of hormone-sensitive lipase in extracts of adipose tissue

Rat adipose tissue was homogenized in 0.154 m KCI, and the supernatant fluid, obtained after centrifugation at 15,000 g, was extracted with benzene to remove triglycerides. Most of the lipase activity in the extracted fluid was precipitated with ammonium sulfate between 15 and 40% saturation. The sp...

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Bibliographic Details
Main Authors: SU-CHEN TSAI, PER BELFRAGE, MARTHA VAUGHAN
Format: Article
Language:English
Published: Elsevier 1970-09-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520429566