Pipeline for large-scale microdroplet bisulfite PCR-based sequencing allows the tracking of hepitype evolution in tumors.

Pipeline for large-scale microdroplet bisulfite PCR-based sequencing allows the tracking of hepitype evolution in tumors.

Cytosine methylation provides an epigenetic level of cellular plasticity that is important for development, differentiation and cancerogenesis. We adopted microdroplet PCR to bisulfite treated target DNA in combination with second generation sequencing to simultaneously assess DNA sequence and methy...

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Bibliographic Details
Main Authors: Alexander Herrmann, Andrea Haake, Ole Ammerpohl, Idoia Martin-Guerrero, Karol Szafranski, Kathryn Stemshorn, Michael Nothnagel, Steve K Kotsopoulos, Julia Richter, Jason Warner, Jeff Olson, Darren R Link, Stefan Schreiber, Michael Krawczak, Matthias Platzer, Peter Nürnberg, Reiner Siebert, Jochen Hampe
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3130030?pdf=render

Internet

http://europepmc.org/articles/PMC3130030?pdf=render

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