Defining the RNA interactome by total RNA‐associated protein purification

Defining the RNA interactome by total RNA‐associated protein purification

Abstract The RNA binding proteome (RBPome) was previously investigated using UV crosslinking and purification of poly(A)‐associated proteins. However, most cellular transcripts are not polyadenylated. We therefore developed total RNA‐associated protein purification (TRAPP) based on 254 nm UV crossli...

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Bibliographic Details
Main Authors: Vadim Shchepachev, Stefan Bresson, Christos Spanos, Elisabeth Petfalski, Lutz Fischer, Juri Rappsilber, David Tollervey
Format: Article
Language:English
Published: Wiley 2019-04-01
Series:Molecular Systems Biology
Subjects:
mass spectrometry
phase separation
protein–RNA interaction
RNA binding sites
yeast
Online Access:https://doi.org/10.15252/msb.20188689

Internet

https://doi.org/10.15252/msb.20188689

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