Variable Expression of Notch1 and Pax5 in Classical Hodgkin Lymphoma and Infection with Epstein–Barr in Pediatric Patients
NOTCH1 and PAX5 participate in the proliferation and differentiation of B and T lymphocytes. Their expression can be modified by activation of NOTCH1, induced by the Epstein–Barr (EBV) viral proteins identified as LMP1 and LMP2. To identify whether PAX5, NOTCH1, and EBV latency genes participate in...
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MDPI AG
2020-06-01
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Online Access: | https://www.mdpi.com/2076-2607/8/6/958 |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Icela Palma-Lara Ana Elena Sánchez-Aldana Elva Jiménez-Hernández Octavio Martínez-Villegas Juan Carlos Núñez-Enríquez Juan Manuel Mejía-Aranguré Sara A. Ochoa Juan Xicohtencatl-Cortes Ariadnna Cruz-Córdova Sergio Zavala-Vega Mariana García-Jiménez Alejandra Contreras-Ramos José Refugio Torres-Nava Guillermo Mora-Ramiro José Arellano-Galindo |
spellingShingle |
Icela Palma-Lara Ana Elena Sánchez-Aldana Elva Jiménez-Hernández Octavio Martínez-Villegas Juan Carlos Núñez-Enríquez Juan Manuel Mejía-Aranguré Sara A. Ochoa Juan Xicohtencatl-Cortes Ariadnna Cruz-Córdova Sergio Zavala-Vega Mariana García-Jiménez Alejandra Contreras-Ramos José Refugio Torres-Nava Guillermo Mora-Ramiro José Arellano-Galindo Variable Expression of Notch1 and Pax5 in Classical Hodgkin Lymphoma and Infection with Epstein–Barr in Pediatric Patients Microorganisms Epstein–Barr virus 1 Hodgkin lymphoma NOTCH1 PAX5 |
author_facet |
Icela Palma-Lara Ana Elena Sánchez-Aldana Elva Jiménez-Hernández Octavio Martínez-Villegas Juan Carlos Núñez-Enríquez Juan Manuel Mejía-Aranguré Sara A. Ochoa Juan Xicohtencatl-Cortes Ariadnna Cruz-Córdova Sergio Zavala-Vega Mariana García-Jiménez Alejandra Contreras-Ramos José Refugio Torres-Nava Guillermo Mora-Ramiro José Arellano-Galindo |
author_sort |
Icela Palma-Lara |
title |
Variable Expression of Notch1 and Pax5 in Classical Hodgkin Lymphoma and Infection with Epstein–Barr in Pediatric Patients |
title_short |
Variable Expression of Notch1 and Pax5 in Classical Hodgkin Lymphoma and Infection with Epstein–Barr in Pediatric Patients |
title_full |
Variable Expression of Notch1 and Pax5 in Classical Hodgkin Lymphoma and Infection with Epstein–Barr in Pediatric Patients |
title_fullStr |
Variable Expression of Notch1 and Pax5 in Classical Hodgkin Lymphoma and Infection with Epstein–Barr in Pediatric Patients |
title_full_unstemmed |
Variable Expression of Notch1 and Pax5 in Classical Hodgkin Lymphoma and Infection with Epstein–Barr in Pediatric Patients |
title_sort |
variable expression of notch1 and pax5 in classical hodgkin lymphoma and infection with epstein–barr in pediatric patients |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-06-01 |
description |
NOTCH1 and PAX5 participate in the proliferation and differentiation of B and T lymphocytes. Their expression can be modified by activation of NOTCH1, induced by the Epstein–Barr (EBV) viral proteins identified as LMP1 and LMP2. To identify whether PAX5, NOTCH1, and EBV latency genes participate in the oncogenic process of pediatric patients with classical Hodgkin lymphoma (cHL), the present study aimed to identify the variable expression of NOTCH1 among disease subtypes and to assess its effect on PAX5 expression. A total of 41 paraffin-embedded tissues from Mexican pediatric patients with cHL were analyzed. The expression of CD30, CD20, NOTCH1, PAX5, and LMP1 was evaluated by immunohistochemistry and immunofluorescence. EBV detection was performed by in situ hybridization. Out of all cases, 78% (32/41) of the cHL cases were EBV positive. NOTCH1 expression was detected in 78.1% (25/32) of EBV-positive cases, nodular sclerosis being the most frequent subtype (11/25, 44%). In cases where the expression of both genes was identified, double immunofluorescence assays were conducted, finding no colocalization. We found that Reed–Sternberg cells had aberrant expression compared to their cells of origin (B lymphocytes) due to the molecular mechanisms involved in the loss of expression of PAX5 and that the identification of NOTCH1 could be considered as a candidate diagnostic/prognostic marker and a therapeutic target in pediatric cHL. |
topic |
Epstein–Barr virus 1 Hodgkin lymphoma NOTCH1 PAX5 |
url |
https://www.mdpi.com/2076-2607/8/6/958 |
work_keys_str_mv |
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doaj-f7fd6cbb3e41459c8c967f4400b744592020-11-25T02:51:23ZengMDPI AGMicroorganisms2076-26072020-06-01895895810.3390/microorganisms8060958Variable Expression of Notch1 and Pax5 in Classical Hodgkin Lymphoma and Infection with Epstein–Barr in Pediatric PatientsIcela Palma-Lara0Ana Elena Sánchez-Aldana1Elva Jiménez-Hernández2Octavio Martínez-Villegas3Juan Carlos Núñez-Enríquez4Juan Manuel Mejía-Aranguré5Sara A. Ochoa6Juan Xicohtencatl-Cortes7Ariadnna Cruz-Córdova8Sergio Zavala-Vega9Mariana García-Jiménez10Alejandra Contreras-Ramos11José Refugio Torres-Nava12Guillermo Mora-Ramiro13José Arellano-Galindo14Molecular and Cellular Morphology Laboratory, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de Mexico 11340, MexicoMolecular and Cellular Morphology Laboratory, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de Mexico 11340, MexicoDepartamento de Hematología Pediátrica, Centro Médico Nacional la Raza, Instituto Mexicano del Seguro Social, Ciudad de Mexico 02990, MexicoDepartamento de Hematología Pediátrica, Centro Médico Nacional la Raza, Instituto Mexicano del Seguro Social, Ciudad de Mexico 02990, MexicoUnidad de Investigación Médica en Epidemiología Clínica, Unidad Médica de Alta Especialidad Hospital de Pediatría “Dr., Silvestre Frenk Freud” Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de Mexico 06600, MexicoCoordinación de Investigación en Salud, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de Mexico 06600, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de Mexico Federico Gómez, Ciudad de Mexico 06720, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de Mexico Federico Gómez, Ciudad de Mexico 06720, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Hospital Infantil de Mexico Federico Gómez, Ciudad de Mexico 06720, MexicoLaboratorio de Neuropatología, Instituto Nacional de neurología Manuel Velasco Suárez, Ciudad de Mexico 14269, MexicoFacultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de Mexico, Ciudad de Mexico 54090, MexicoBiology Development Department, Hospital Infantil de Mexico Federico Gómez, Ciudad de Mexico 06720, MexicoServicio de Oncohematología, Hospital Pediátrico Moctezuma, Secretaría de Salud del Gobierno de la Ciudad de Mexico 15530, MexicoLaboratorio de Virología Clínica y Experimental, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de Mexico Federico Gómez, Ciudad de Mexico 06720, MexicoLaboratorio de Virología Clínica y Experimental, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de Mexico Federico Gómez, Ciudad de Mexico 06720, MexicoNOTCH1 and PAX5 participate in the proliferation and differentiation of B and T lymphocytes. Their expression can be modified by activation of NOTCH1, induced by the Epstein–Barr (EBV) viral proteins identified as LMP1 and LMP2. To identify whether PAX5, NOTCH1, and EBV latency genes participate in the oncogenic process of pediatric patients with classical Hodgkin lymphoma (cHL), the present study aimed to identify the variable expression of NOTCH1 among disease subtypes and to assess its effect on PAX5 expression. A total of 41 paraffin-embedded tissues from Mexican pediatric patients with cHL were analyzed. The expression of CD30, CD20, NOTCH1, PAX5, and LMP1 was evaluated by immunohistochemistry and immunofluorescence. EBV detection was performed by in situ hybridization. Out of all cases, 78% (32/41) of the cHL cases were EBV positive. NOTCH1 expression was detected in 78.1% (25/32) of EBV-positive cases, nodular sclerosis being the most frequent subtype (11/25, 44%). In cases where the expression of both genes was identified, double immunofluorescence assays were conducted, finding no colocalization. We found that Reed–Sternberg cells had aberrant expression compared to their cells of origin (B lymphocytes) due to the molecular mechanisms involved in the loss of expression of PAX5 and that the identification of NOTCH1 could be considered as a candidate diagnostic/prognostic marker and a therapeutic target in pediatric cHL.https://www.mdpi.com/2076-2607/8/6/958Epstein–Barr virus 1Hodgkin lymphomaNOTCH1PAX5 |