A high-throughput platform for lentiviral overexpression screening of the human ORFeome.

In response to the growing need for functional analysis of the human genome, we have developed a platform for high-throughput functional screening of genes overexpressed from lentiviral vectors. Protein-coding human open reading frames (ORFs) from the Mammalian Gene Collection were transferred into...

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Bibliographic Details
Main Authors: Dubravka Škalamera, Max V Ranall, Benjamin M Wilson, Paul Leo, Amy S Purdon, Carolyn Hyde, Ehsan Nourbakhsh, Sean M Grimmond, Simon C Barry, Brian Gabrielli, Thomas J Gonda
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3101218?pdf=render