| Summary: | <i>Streptomyces albus</i> J1074 is recognized as an effective host for heterologous production of natural products. Its fast growth and efficient genetic toolbox due to a naturally minimized genome have contributed towards its advantage in expressing biosynthetic pathways for a diverse repertoire of products such as antibiotics and flavonoids. In order to develop precise model-driven engineering strategies for de novo production of natural products, a genome-scale metabolic model (GEM) was reconstructed for the microorganism based on protein homology to model species <i>Streptomyces</i> <i>coelicolor</i> while drawing annotated data from databases and literature for further curation. To demonstrate its capabilities, the <i>Salb</i>-GEM was used to predict overexpression targets for desirable compounds using flux scanning with enforced objective function (FSEOF). <i>Salb</i>-GEM was also utilized to investigate the effect of a minimized genome on metabolic gene essentialities in comparison to another <i>Streptomyces</i> species, <i>S. coelicolor</i>.
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