Yeast Ded1 promotes 48S translation pre-initiation complex assembly in an mRNA-specific and eIF4F-dependent manner
DEAD-box RNA helicase Ded1 is thought to resolve secondary structures in mRNA 5'-untranslated regions (5'-UTRs) that impede 48S preinitiation complex (PIC) formation at the initiation codon. We reconstituted Ded1 acceleration of 48S PIC assembly on native mRNAs in a pure system, and recapi...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2018-10-01
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Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/38892 |