New methods of removing debris and high-throughput counting of cyst nematode eggs extracted from field soil.

The soybean cyst nematode (SCN), Heterodera glycines, is the most damaging pathogen of soybeans in the United States. To assess the severity of nematode infestations in the field, SCN egg population densities are determined. Cysts (dead females) of the nematode must be extracted from soil samples an...

Full description

Bibliographic Details
Main Authors: Upender Kalwa, Christopher Legner, Elizabeth Wlezien, Gregory Tylka, Santosh Pandey
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0223386