Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits

Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits

Purpose: Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. Methods: Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse IgG2b. Sodium citrate buffer (0.1 M, pH: 3.5)...

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Bibliographic Details
Main Authors: Sadeq Eivazi, Jafar Majidi, Leili Aghebati Maleki, Jalal Abdolalizadeh, Mehdi Yousefi, Majid Ahmadi, Somayeh Dadashi, Zahra Moradi, Elmira Zolali
Format: Article
Language:English
Published: Tabriz University of Medical Sciences 2015-03-01
Series:Advanced Pharmaceutical Bulletin
Subjects:
Mouse IgG2b
Affinity chromatography
Ion-exchange chromatography
Horse radish peroxidase conjugation
Online Access:http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol5-2015/15-Majidi.pdf

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http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol5-2015/15-Majidi.pdf

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