Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador
Abstract Background “Horchata” is an herbal mixture infusion consumed in Southern Ecuador; 66% of its plants are anti-inflammatory medicinal plant, and 51% are analgesics. Anti-inflammatory substances can prevent carcinogenesis mediated by cytotoxic effects and can prevent DNA damage. The aim of thi...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2017-12-01
|
Series: | BMC Complementary and Alternative Medicine |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12906-017-2048-x |
id |
doaj-fa7fa492efd647119542f6c662cc26bb |
---|---|
record_format |
Article |
spelling |
doaj-fa7fa492efd647119542f6c662cc26bb2020-11-25T02:02:49ZengBMCBMC Complementary and Alternative Medicine1472-68822017-12-0117111410.1186/s12906-017-2048-xCytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South EcuadorNatalia Bailon-Moscoso0Fani Tinitana1Ruth Martínez-Espinosa2Andrea Jaramillo-Velez3Alejandra Palacio-Arpi4Jessica Aguilar-Hernandez5Juan Carlos Romero-Benavides6Departamento de Ciencias de la Salud, Universidad Técnica Particular de LojaDepartamento de Ciencias Biológicas, Universidad Técnica Particular de LojaDepartamento de Química y Ciencias Exactas, Universidad Técnica Particular de LojaDepartamento de Ciencias de la Salud, Universidad Técnica Particular de LojaDepartamento de Ciencias de la Salud, Universidad Técnica Particular de LojaDepartamento de Ciencias de la Salud, Universidad Técnica Particular de LojaDepartamento de Química y Ciencias Exactas, Universidad Técnica Particular de LojaAbstract Background “Horchata” is an herbal mixture infusion consumed in Southern Ecuador; 66% of its plants are anti-inflammatory medicinal plant, and 51% are analgesics. Anti-inflammatory substances can prevent carcinogenesis mediated by cytotoxic effects and can prevent DNA damage. The aim of this study was to evaluate the cytotoxicity and apoptotic/antigenotoxic effects of horchata as well as its mechanism. Methods Nine different varieties of horchata were prepared in the traditional way and then freeze-dried. Phytochemical screening tested for the presence of secondary metabolites using standard procedures and antioxidant activities. The cytotoxic activity was evaluated on cerebral astrocytoma (D-384), prostate cancer (PC-3), breast cancer (MCF-7), colon cancer (RKO), lung cancer (A-549), immortalized Chinese hamster ovary cells (CHO-K1), and human peripheral blood lymphocytes via a MTS assay. The pro-apoptotic effects were evaluated with Anexin V/Propidium Iodide and western blot of Bax, Bcl-2, TP53, and TP73. Induction and reduction of ROS were assessed by fluorimetry. Genotoxic and antigenotoxic effects were evaluated with a comet assay and micronuclei on binucleated cells. Results Five of nine horchatas had cytotoxic effects against D-384 while not affecting normal cells. These horchatas induce cell death by apoptosis modulated by p53/p73. In CHO-K1 cells, the horchatas decrease the damage induced by hydrogen peroxide and Mitomycin C measured in the comet and micronucleus assay respectively. Conclusions The IC50 range of effective horchatas in D-384 was 41 to 122 μg·mL−1. This effect may be related to its use in traditional medicine (brain tonic). On the other hand, immortalized Chinese hamster ovary cells (CHO-K1) and lymphocytes did not show a cytotoxic effect. The most potent horchata induced apoptosis via a p53/p73-mediated mechanism. The horchatas present antigenotoxic properties, which may be related to the antioxidant capacity. Future studies on horchata components are necessary to understand the interactions and beneficial properties.http://link.springer.com/article/10.1186/s12906-017-2048-xHorchataCancerAntigenotoxicityAnticlastogenicApoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Natalia Bailon-Moscoso Fani Tinitana Ruth Martínez-Espinosa Andrea Jaramillo-Velez Alejandra Palacio-Arpi Jessica Aguilar-Hernandez Juan Carlos Romero-Benavides |
spellingShingle |
Natalia Bailon-Moscoso Fani Tinitana Ruth Martínez-Espinosa Andrea Jaramillo-Velez Alejandra Palacio-Arpi Jessica Aguilar-Hernandez Juan Carlos Romero-Benavides Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador BMC Complementary and Alternative Medicine Horchata Cancer Antigenotoxicity Anticlastogenic Apoptosis |
author_facet |
Natalia Bailon-Moscoso Fani Tinitana Ruth Martínez-Espinosa Andrea Jaramillo-Velez Alejandra Palacio-Arpi Jessica Aguilar-Hernandez Juan Carlos Romero-Benavides |
author_sort |
Natalia Bailon-Moscoso |
title |
Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador |
title_short |
Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador |
title_full |
Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador |
title_fullStr |
Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador |
title_full_unstemmed |
Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador |
title_sort |
cytotoxic, antioxidative, genotoxic and antigenotoxic effects of horchata, beverage of south ecuador |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2017-12-01 |
description |
Abstract Background “Horchata” is an herbal mixture infusion consumed in Southern Ecuador; 66% of its plants are anti-inflammatory medicinal plant, and 51% are analgesics. Anti-inflammatory substances can prevent carcinogenesis mediated by cytotoxic effects and can prevent DNA damage. The aim of this study was to evaluate the cytotoxicity and apoptotic/antigenotoxic effects of horchata as well as its mechanism. Methods Nine different varieties of horchata were prepared in the traditional way and then freeze-dried. Phytochemical screening tested for the presence of secondary metabolites using standard procedures and antioxidant activities. The cytotoxic activity was evaluated on cerebral astrocytoma (D-384), prostate cancer (PC-3), breast cancer (MCF-7), colon cancer (RKO), lung cancer (A-549), immortalized Chinese hamster ovary cells (CHO-K1), and human peripheral blood lymphocytes via a MTS assay. The pro-apoptotic effects were evaluated with Anexin V/Propidium Iodide and western blot of Bax, Bcl-2, TP53, and TP73. Induction and reduction of ROS were assessed by fluorimetry. Genotoxic and antigenotoxic effects were evaluated with a comet assay and micronuclei on binucleated cells. Results Five of nine horchatas had cytotoxic effects against D-384 while not affecting normal cells. These horchatas induce cell death by apoptosis modulated by p53/p73. In CHO-K1 cells, the horchatas decrease the damage induced by hydrogen peroxide and Mitomycin C measured in the comet and micronucleus assay respectively. Conclusions The IC50 range of effective horchatas in D-384 was 41 to 122 μg·mL−1. This effect may be related to its use in traditional medicine (brain tonic). On the other hand, immortalized Chinese hamster ovary cells (CHO-K1) and lymphocytes did not show a cytotoxic effect. The most potent horchata induced apoptosis via a p53/p73-mediated mechanism. The horchatas present antigenotoxic properties, which may be related to the antioxidant capacity. Future studies on horchata components are necessary to understand the interactions and beneficial properties. |
topic |
Horchata Cancer Antigenotoxicity Anticlastogenic Apoptosis |
url |
http://link.springer.com/article/10.1186/s12906-017-2048-x |
work_keys_str_mv |
AT nataliabailonmoscoso cytotoxicantioxidativegenotoxicandantigenotoxiceffectsofhorchatabeverageofsouthecuador AT fanitinitana cytotoxicantioxidativegenotoxicandantigenotoxiceffectsofhorchatabeverageofsouthecuador AT ruthmartinezespinosa cytotoxicantioxidativegenotoxicandantigenotoxiceffectsofhorchatabeverageofsouthecuador AT andreajaramillovelez cytotoxicantioxidativegenotoxicandantigenotoxiceffectsofhorchatabeverageofsouthecuador AT alejandrapalacioarpi cytotoxicantioxidativegenotoxicandantigenotoxiceffectsofhorchatabeverageofsouthecuador AT jessicaaguilarhernandez cytotoxicantioxidativegenotoxicandantigenotoxiceffectsofhorchatabeverageofsouthecuador AT juancarlosromerobenavides cytotoxicantioxidativegenotoxicandantigenotoxiceffectsofhorchatabeverageofsouthecuador |
_version_ |
1724951246631075840 |