NMR Structure and Dynamics of TonB Investigated by Scar-Less Segmental Isotopic Labeling Using a Salt-Inducible Split Intein

NMR Structure and Dynamics of TonB Investigated by Scar-Less Segmental Isotopic Labeling Using a Salt-Inducible Split Intein

The growing understanding of partially unfolded proteins increasingly points to their biological relevance in allosteric regulation, complex formation, and protein design. However, the structural characterization of disordered proteins remains challenging. NMR methods can access both the dynamics an...

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Bibliographic Details
Main Authors: Annika Ciragan, Sofia M. Backlund, Kornelia M. Mikula, Hannes M. Beyer, O. H. Samuli Ollila, Hideo Iwaï
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-03-01
Series:Frontiers in Chemistry
Subjects:
inteins
protein ligation
NMR spectroscopy
segmental isotopic labeling
TonB
intrinsically disordered protein
Online Access:https://www.frontiersin.org/article/10.3389/fchem.2020.00136/full

Internet

https://www.frontiersin.org/article/10.3389/fchem.2020.00136/full

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