Strengths and Weaknesses of Recently Engineered Red Fluorescent Proteins Evaluated in Live Cells Using Fluorescence Correlation Spectroscopy

Strengths and Weaknesses of Recently Engineered Red Fluorescent Proteins Evaluated in Live Cells Using Fluorescence Correlation Spectroscopy

The scientific community is still looking for a bright, stable red fluorescent protein (FP) as functional as the current best derivatives of green fluorescent protein (GFP). The red FPs exploit the reduced background of cells imaged in the red region of the visible spectrum, but photophysical short...

Full description

Bibliographic Details
Main Authors: Richard N. Day, Michael W. Davidson, Amanda P. Siegel, Michelle A. Baird
Format: Article
Language:English
Published: MDPI AG 2013-10-01
Series:International Journal of Molecular Sciences
Subjects:
fluorescent protein
fluorescent correlation spectroscopy (FCS)
diffusion
intrinsic brightness
flickering
Online Access:http://www.mdpi.com/1422-0067/14/10/20340

Internet

http://www.mdpi.com/1422-0067/14/10/20340

Similar Items