Expanding the Zebrafish Genetic Code through Site-Specific Introduction of Azido-lysine, Bicyclononyne-lysine, and Diazirine-lysine

Expanding the Zebrafish Genetic Code through Site-Specific Introduction of Azido-lysine, Bicyclononyne-lysine, and Diazirine-lysine

Site-specific incorporation of un-natural amino acids (UNAA) is a powerful approach to engineer and understand protein function. Site-specific incorporation of UNAAs is achieved through repurposing the amber codon (UAG) as a sense codon for the UNAA, using a tRNA<sup>CUA</sup> that base...

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Bibliographic Details
Main Authors: Junetha Syed, Saravanan Palani, Scott T. Clarke, Zainab Asad, Andrew R. Bottrill, Alexandra M.E. Jones, Karuna Sampath, Mohan K. Balasubramanian
Format: Article
Language:English
Published: MDPI AG 2019-05-01
Series:International Journal of Molecular Sciences
Subjects:
genetic code expansion
zebrafish
unnatural amino acid
protein engineering
proteomics
crosslinking
click chemistry
Online Access:https://www.mdpi.com/1422-0067/20/10/2577

Internet

https://www.mdpi.com/1422-0067/20/10/2577

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