Use of real-time cellular analysis and Plackett-Burman design to develop the serum-free media for PC-3 prostate cancer cells.

In this study, we developed a rapid strategy to screen a serum-free medium for culturing the anchorage-dependent PC-3 prostate cancer cells, which was going to be prepared in large scale to generate GM-CSF/TNFα-surface-modified whole cell prostate cancer vaccine. Automated real-time cellular analysi...

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Bibliographic Details
Main Authors: Ai Zhao, Fahai Chen, Chunhong Ning, Haiming Wu, Huanfang Song, Yanqing Wu, Rong Chen, Kaihua Zhou, Xiaoling Xu, Yinxiang Lu, Jimin Gao
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5612757?pdf=render