The use of coded PCR primers enables high-throughput sequencing of multiple homolog amplification products by 454 parallel sequencing.

The invention of the Genome Sequence 20 DNA Sequencing System (454 parallel sequencing platform) has enabled the rapid and high-volume production of sequence data. Until now, however, individual emulsion PCR (emPCR) reactions and subsequent sequencing runs have been unable to combine template DNA fr...

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Bibliographic Details
Main Authors: Jonas Binladen, M Thomas P Gilbert, Jonathan P Bollback, Frank Panitz, Christian Bendixen, Rasmus Nielsen, Eske Willerslev
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2007-02-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC1797623?pdf=render