Creation of Functional Viruses from Non-Functional cDNA Clones Obtained from an RNA Virus Population by the Use of Ancestral Reconstruction.

Creation of Functional Viruses from Non-Functional cDNA Clones Obtained from an RNA Virus Population by the Use of Ancestral Reconstruction.

RNA viruses have the highest known mutation rates. Consequently it is likely that a high proportion of individual RNA virus genomes, isolated from an infected host, will contain lethal mutations and be non-functional. This is problematic if the aim is to clone and investigate high-fitness, functiona...

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Bibliographic Details
Main Authors: Ulrik Fahnøe, Anders Gorm Pedersen, Carolin Dräger, Richard J Orton, Sandra Blome, Dirk Höper, Martin Beer, Thomas Bruun Rasmussen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4613144?pdf=render

Internet

http://europepmc.org/articles/PMC4613144?pdf=render

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