Validation of a multiplex reverse transcription and pre-amplification method using TaqMan® MicroRNA assays.
AbstractSince the discovery of microRNAs (miRNAs), different approaches have been developed to label, amplify and quantify miRNAs. The TaqMan® technology, provided by Applied Biosystems, uses a stem-loop RT primer system to reverse transcribe the RNA and amplify the cDNA. This method is widely used...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-11-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00413/full |