Structural analysis of microRNA-target interaction by sequential seed mutagenesis and stem-loop 3' RACE.

BACKGROUND: As a consequence of recent RNAseq efforts, miRNAomes of diverse tissues and species are available. However, most interactions between microRNAs and regulated mRNAs are still to be deciphered. While in silico analysis of microRNAs results in prediction of hundreds of potential targets, bo...

Full description

Bibliographic Details
Main Authors: Marc Bohmer, Jutta Sharbati, Jennifer Zur Bruegge, Ralf Einspanier, Soroush Sharbati
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3839922?pdf=render