Periplasmic Nanobody-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among <i>Marburgvirus</i> and <i>Dianlovirus</i>

Periplasmic Nanobody-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among <i>Marburgvirus</i> and <i>Dianlovirus</i>

We explore evolved soybean ascorbate peroxidase (APEX2) as a reporter when fused to the C-termini of llama nanobodies (single-domain antibodies, sdAb; variable domains of heavy chain-only antibodies, VHH) targeted to the <i>E. coli</i> periplasm. Periplasmic expression preserves authenti...

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Bibliographic Details
Main Authors: Laura J. Sherwood, Andrew Hayhurst
Format: Article
Language:English
Published: MDPI AG 2019-04-01
Series:Viruses
Subjects:
peroxidase
APEX2
VHH
sdAb
nanobody
Filovirus
Ebola
Marburg
nucleoprotein
nanoluciferase
Online Access:https://www.mdpi.com/1999-4915/11/4/364

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https://www.mdpi.com/1999-4915/11/4/364

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