CRISPR-Cas9 Gene Editing in Lizards through Microinjection of Unfertilized Oocytes

Summary: CRISPR-Cas9-mediated gene editing has enabled the direct manipulation of gene function in many species. However, the reproductive biology of reptiles presents unique barriers for the use of this technology, and there are no reptiles with effective methods for targeted mutagenesis. Here, we...

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Bibliographic Details
Main Authors: Ashley M. Rasys, Sungdae Park, Rebecca E. Ball, Aaron J. Alcala, James D. Lauderdale, Douglas B. Menke
Format: Article
Language:English
Published: Elsevier 2019-08-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124719310058