New variants in NLRP3 inflammasome genes increase risk for asthma and Blomia tropicalis-induced allergy in a Brazilian population
Atopic asthma is a chronic lung disease of lower airways caused mainly due to action of T-helper (Th) 2 type cytokines, eosinophilic inflammation, mucus hypersecretion and airway remodelling. Interleukin (IL)-33 increases type 2 immunity polarization in airway playing critical role in eosinophilic a...
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2020-09-01
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doaj-fbd34ccbbe6f4ad09da1ec3c26a1f2fa2020-11-25T03:30:30ZengElsevierCytokine: X2590-15322020-09-0123100032New variants in NLRP3 inflammasome genes increase risk for asthma and Blomia tropicalis-induced allergy in a Brazilian populationGerson de A. Queiroz0Raimon R. da Silva1Anaque de O. Pires2Ryan dos S. Costa3Neuza M. Alcântara-Neves4Thiago M. da Silva5Mauricio L. Barreto6Sergio C. Oliveira7Camila A. Figueirêdo8Instituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Salvador, BrazilInstituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Salvador, BrazilInstituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Salvador, BrazilInstituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Salvador, BrazilInstituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Salvador, BrazilDepartamento de Ciências Biológicas, Universidade Estadual do Sudoeste da Bahia, Jequié, Bahia, BrazilCentro de Integração de Dados e Conhecimento para Saúde (CIDACS), Fiocruz, Salvador, Bahia, Brazil; Instituto de Saude Coletiva, Universidade Federal da Bahia, Salvador, BrazilInstituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BrazilInstituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Salvador, Brazil; Corresponding author at: Laboratório de Imunofarmacologia e Biologia Molecular – Universidade Federal da Bahia, Avenida Reitor Miguel Calmon, s/n, CEP: 41.110-100, Salvador, Brazil.Atopic asthma is a chronic lung disease of lower airways caused mainly due to action of T-helper (Th) 2 type cytokines, eosinophilic inflammation, mucus hypersecretion and airway remodelling. Interleukin (IL)-33 increases type 2 immunity polarization in airway playing critical role in eosinophilic asthma. On the other hand, NLRP3 inflammasome activation results in the release of caspase-1 (Casp-1) which, in its turn, promotes IL-33 inactivation. Recent studies have shown associations between NLRP3 variants and inflammatory diseases. However, no study with genes in NLRP3 inflammassome route has been conducted so far with asthma and atopy in any population to date. Blood samples were collected from 1246 asthmatic and non-asthmatic children. Associations were tested for single nucleotide polymorphism (SNP)s in NLRP3 and CASP1 with asthma and markers of atopy and in cultures stimulated with Blomia tropicalis (Bt) mite crude extract. The T allele of rs4925648 (NLRP3) was associated with increased asthma risk (OR 1.50, P = 0.005). In addition, the T allele of rs12130711 polymorphism, whithin the same gene, acted as a protector factor for asthma (OR 0.78, P = 0.038). On the other hand, the C allele of rs4378247 NLRP3 variant was associated with lower levels of IL-13 production when peripheral blood cells were stimulated with Bt (OR 0.39, P = 4E-04). In addition, the greater the number of risk alleles in IL33/NLRP3/CASP1 route the greater was the risk for asthma. The T allele of rs7925706 CASP1 variant was also associated with increased risk for asthma (OR 1.47, P = 0.008). In addition, this same allele increased the eosinophil counts in blood (mm3) in asthmatic individuals compared with non-asthmatic (P = 0.0004). These results suggest that NLRP3 and CASP1 polymorphisms may be associated with susceptibility for asthma and markers of atopy in our population.http://www.sciencedirect.com/science/article/pii/S2590153220300124AsthmaAllergyInflammasomeVariantsNLRP3CASP1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gerson de A. Queiroz Raimon R. da Silva Anaque de O. Pires Ryan dos S. Costa Neuza M. Alcântara-Neves Thiago M. da Silva Mauricio L. Barreto Sergio C. Oliveira Camila A. Figueirêdo |
spellingShingle |
Gerson de A. Queiroz Raimon R. da Silva Anaque de O. Pires Ryan dos S. Costa Neuza M. Alcântara-Neves Thiago M. da Silva Mauricio L. Barreto Sergio C. Oliveira Camila A. Figueirêdo New variants in NLRP3 inflammasome genes increase risk for asthma and Blomia tropicalis-induced allergy in a Brazilian population Cytokine: X Asthma Allergy Inflammasome Variants NLRP3 CASP1 |
author_facet |
Gerson de A. Queiroz Raimon R. da Silva Anaque de O. Pires Ryan dos S. Costa Neuza M. Alcântara-Neves Thiago M. da Silva Mauricio L. Barreto Sergio C. Oliveira Camila A. Figueirêdo |
author_sort |
Gerson de A. Queiroz |
title |
New variants in NLRP3 inflammasome genes increase risk for asthma and Blomia tropicalis-induced allergy in a Brazilian population |
title_short |
New variants in NLRP3 inflammasome genes increase risk for asthma and Blomia tropicalis-induced allergy in a Brazilian population |
title_full |
New variants in NLRP3 inflammasome genes increase risk for asthma and Blomia tropicalis-induced allergy in a Brazilian population |
title_fullStr |
New variants in NLRP3 inflammasome genes increase risk for asthma and Blomia tropicalis-induced allergy in a Brazilian population |
title_full_unstemmed |
New variants in NLRP3 inflammasome genes increase risk for asthma and Blomia tropicalis-induced allergy in a Brazilian population |
title_sort |
new variants in nlrp3 inflammasome genes increase risk for asthma and blomia tropicalis-induced allergy in a brazilian population |
publisher |
Elsevier |
series |
Cytokine: X |
issn |
2590-1532 |
publishDate |
2020-09-01 |
description |
Atopic asthma is a chronic lung disease of lower airways caused mainly due to action of T-helper (Th) 2 type cytokines, eosinophilic inflammation, mucus hypersecretion and airway remodelling. Interleukin (IL)-33 increases type 2 immunity polarization in airway playing critical role in eosinophilic asthma. On the other hand, NLRP3 inflammasome activation results in the release of caspase-1 (Casp-1) which, in its turn, promotes IL-33 inactivation. Recent studies have shown associations between NLRP3 variants and inflammatory diseases. However, no study with genes in NLRP3 inflammassome route has been conducted so far with asthma and atopy in any population to date. Blood samples were collected from 1246 asthmatic and non-asthmatic children. Associations were tested for single nucleotide polymorphism (SNP)s in NLRP3 and CASP1 with asthma and markers of atopy and in cultures stimulated with Blomia tropicalis (Bt) mite crude extract. The T allele of rs4925648 (NLRP3) was associated with increased asthma risk (OR 1.50, P = 0.005). In addition, the T allele of rs12130711 polymorphism, whithin the same gene, acted as a protector factor for asthma (OR 0.78, P = 0.038). On the other hand, the C allele of rs4378247 NLRP3 variant was associated with lower levels of IL-13 production when peripheral blood cells were stimulated with Bt (OR 0.39, P = 4E-04). In addition, the greater the number of risk alleles in IL33/NLRP3/CASP1 route the greater was the risk for asthma. The T allele of rs7925706 CASP1 variant was also associated with increased risk for asthma (OR 1.47, P = 0.008). In addition, this same allele increased the eosinophil counts in blood (mm3) in asthmatic individuals compared with non-asthmatic (P = 0.0004). These results suggest that NLRP3 and CASP1 polymorphisms may be associated with susceptibility for asthma and markers of atopy in our population. |
topic |
Asthma Allergy Inflammasome Variants NLRP3 CASP1 |
url |
http://www.sciencedirect.com/science/article/pii/S2590153220300124 |
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