Primary mouse osteoblast and osteoclast culturing and analysis

Summary: Mesenchymal-derived osteoblasts play a key role in bone formation via synthesis and mineralization of the bone and bone remodeling. Osteoclasts are multinucleated cells of hematopoietic origin with a role in bone resorption. Here, we describe a protocol for generating primary cultures of th...

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Bibliographic Details
Main Authors: Claire Chevalier, Melis Çolakoğlu, Julia Brun, Cyril Thouverey, Nicolas Bonnet, Serge Ferrari, Mirko Trajkovski
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001593